3OJV
Crystal Structure of FGF1 complexed with the ectodomain of FGFR1c exhibiting an ordered ligand specificity-determining betaC'-betaE loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.324, 53.421, 80.463 |
| Unit cell angles | 106.44, 106.40, 94.44 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1evt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Number of reflections | 24626 | ||
| <I/σ(I)> | 16.6 | 49.8 | 5.5 |
| Completeness [%] | 97.8 | 98.9 | 91 |
| Redundancy | 3.7 | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris, 15% PEG4000, 0.1M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
