3OGO

Structure of the GFP:GFP-nanobody complex at 2.8 A resolution in spacegroup P21212

Replaces:  3MIQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	140.100, 147.600, 101.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.432 - 2.800
R-factor	0.2043
Rwork	0.202
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.175
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.430	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.080
Number of reflections	48206
<I/σ(I)>		2.9
Completeness [%]	91.8	73
Redundancy	4.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	20% PEG 4000, 20% isopropanol, 0.1M trisodium citrate dihydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K