3O78
The structure of Ca2+ Sensor (Case-12)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.374, 101.635, 82.211 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 50.830 - 2.600 |
| R-factor | 0.24546 |
| Rwork | 0.242 |
| R-free | 0.31614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o77 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.726 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 23464 | |
| <I/σ(I)> | 12 | 2.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Reservoir: 100mM Tris HCL (pH 5.5), 100mM (NH4)2SO4, 21% PEG 3350; Protein stock: 7.6 mg/ml Protein, 50mM Tris HCl (pH 7.4), 150mM NaCl; Seed stock solution: 20mM CaCl2, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
