3O4O
Crystal structure of an Interleukin-1 receptor complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.830, 177.470, 180.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.585 - 3.300 |
R-factor | 0.2534 |
Rwork | 0.252 |
R-free | 0.28920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i1b |
RMSD bond length | 0.012 |
RMSD bond angle | 1.120 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 22655 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 17% PEG 10000, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |