3NH2
Crystal structure of RNase T in complex with a stem DNA with a 3' overhang
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 |
Unit cell lengths | 60.379, 62.560, 62.657 |
Unit cell angles | 82.77, 82.92, 66.13 |
Refinement procedure
Resolution | 26.761 - 2.300 |
R-factor | 0.19 |
Rwork | 0.186 |
R-free | 0.24090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngy |
RMSD bond length | 0.003 |
RMSD bond angle | 0.736 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 35579 | |
<I/σ(I)> | 9.7 | 2.4 |
Completeness [%] | 96.5 | 96.5 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1% w/v n-Octyl-beta-D-glucoside, 0.1M Sodium citrate tribasic dihydrate, pH 5.5, 22% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |