3NH2

Crystal structure of RNase T in complex with a stem DNA with a 3' overhang

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F	Ribonuclease T	polymer	235	25719.0	4	UniProt (P30014) Pfam (PF00929) In PDB	Escherichia coli	RNase T, Exoribonuclease T
2	C, D, G, H	5'-D(P*TP*TP*AP*CP*AP*AP*C)-3'	polymer	7	2081.4	4		synthetic construct
3		water	water		18.0	345	Chemie (HOH)

Sequence modifications

A, B, E, F: 1 - 215 (UniProt: P30014)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	111201.8
All*	Total formula weight	111201.8

*Water molecules are not included.