3NGZ
Crystal structure of RNase T in complex with a non-preferred ssDNA (GC) with one Mg in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.747, 105.585, 47.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.459 - 2.100 |
| R-factor | 0.1998 |
| Rwork | 0.195 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.985 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 28759 | |
| <I/σ(I)> | 18.6 | 5 |
| Completeness [%] | 97.9 | 99.6 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 18% v/v 2-Propanol, 0.1M Sodium citrate tribasic dihydrate pH 5.5, 20% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
