3NGZ

Crystal structure of RNase T in complex with a non-preferred ssDNA (GC) with one Mg in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribonuclease T	polymer	235	25647.0	2	UniProt (P30014) Pfam (PF00929)	Escherichia coli	RNase T, Exoribonuclease T
2	C, D (C, D)	5'-D(P*GP*C)-3'	polymer	2	573.4	2
3	E, G (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, H (A, B)	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
5	I, J, K, L (A, B, C, D)	water	water		18.0	584	Chemie (HOH)

Sequence modifications

A, B: 1 - 215 (UniProt: P30014)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 92	Glu 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52440.8
Non-Polymers*	Number of molecules	4
	Total formula weight	166.5
All*	Total formula weight	52607.3

*Water molecules are not included.