3NG8
Crystal structure of an abridged SER TO ALA MUTANT OF THE MATURE ECTODOMAIN of the human receptor-type protein-tyrosine phosphatase ICA512/IA-2 at PH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.554, 66.544, 73.712 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.450 - 1.350 |
| R-factor | 0.17934 |
| Rwork | 0.177 |
| R-free | 0.22394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qt7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.039 | 0.280 |
| Number of reflections | 34457 | |
| <I/σ(I)> | 44.2 | 3 |
| Completeness [%] | 98.4 | 85.5 |
| Redundancy | 5.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 30% (W/V) PEG 4000, 0.1 M TrisCl PH 8.5, 0.2 M CACL2, VAPOR DIFFUSION, HANGING DROP, temperature 292 |
