3NDH
Restriction endonuclease in complex with substrate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.310, 72.910, 88.760 |
Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
Resolution | 19.510 - 1.300 |
R-factor | 0.1723 |
Rwork | 0.172 |
R-free | 0.18318 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.261 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.074 | 0.266 |
Number of reflections | 118539 | |
<I/σ(I)> | 5 | 2.8 |
Completeness [%] | 98.7 | 99.4 |
Redundancy | 2.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 0.1M sodium acetate PH 4.5, 200MM, ammonium sulfate, 30% PEG400, 1.5% 1,2,3-heptanetriol, VAPOR, DIFFUSION, SITTING DROP, TEMPERATURE 294K, VAPOR DIFFUSION, HANGING DROP |