3N50
Human Early B-cell factor 3 (EBF3) IPT/TIG and HLHLH domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-26 |
| Detector | X-flash |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 229.640, 229.640, 229.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.838 - 3.102 |
| R-factor | 0.1904 |
| Rwork | 0.188 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mqi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.068 | 0.700 |
| Number of reflections | 36262 | |
| <I/σ(I)> | 20.1 | 2.55 |
| Completeness [%] | 20.0 | |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 2.9M sodium chloride 0.1M BTP, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
