3MOP
The ternary Death Domain complex of MyD88, IRAK4, and IRAK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.709, 307.116, 187.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.958 - 3.400 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26100 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.588 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.410 |
| High resolution limit [Å] | 3.300 | 7.310 | 3.300 |
| Rmerge | 0.079 | 0.042 | 0.546 |
| Number of reflections | 39643 | ||
| <I/σ(I)> | 17 | ||
| Completeness [%] | 94.3 | 97.6 | 61.8 |
| Redundancy | 9.1 | 10.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100-250 mM MgCl2, 8-15 % ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
