3LBL
Structure of human MDM2 protein in complex with Mi-63-analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2009-07-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.940, 97.350, 106.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.19285 |
Rwork | 0.191 |
R-free | 0.23606 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ycr |
RMSD bond length | 0.007 |
RMSD bond angle | 1.737 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.044 | 0.184 |
Number of reflections | 39422 | |
<I/σ(I)> | 20.45 | 5.5 |
Completeness [%] | 83.0 | 42.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 300 | 0.1M sodium-acetate-trihydrate, 2M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |