3LAW
Structure of GTP-bound L129F mutant Rab7
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.554, 89.335, 89.378 |
| Unit cell angles | 71.24, 85.97, 85.91 |
Refinement procedure
| Resolution | 19.880 - 2.800 |
| R-factor | 0.23678 |
| Rwork | 0.220 |
| R-free | 0.27265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.871 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 23869 | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 291 | 0.2 M sodium formate, 10mM trimethylamine hydrochloride, and 20% polyethylene glycol 3,350, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
