3KXU
Crystal structure of human ferritin FTL498InsTC pathogenic mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 151.355, 151.355, 151.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.780 - 1.850 |
| R-factor | 0.18031 |
| Rwork | 0.179 |
| R-free | 0.21078 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ffx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.244 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.780 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.407 |
| Number of reflections | 25503 | |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.25M CADMIUM SULFATE, 0.1M HEPES, 0.96M SODIUM ACETATE, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
