3KUC
Complex of Rap1A(E30D/K31E)GDP with RafRBD(A85K/N71R)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00472 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.120, 68.540, 101.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.500 - 1.920 |
| R-factor | 0.17559 |
| Rwork | 0.174 |
| R-free | 0.20893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gua |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.171 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.840 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.046 | 0.196 |
| Number of reflections | 23432 | |
| <I/σ(I)> | 27.27 | 7.52 |
| Completeness [%] | 98.5 | 95.3 |
| Redundancy | 7.71 | 7.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20-25% PEG 8000, 100 mM Tris or HEPES pH 7.2-7.6, 10-200 mM Ca Acetate or 100 mM Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
