3KTM

Structure of the Heparin-induced E1-Dimer of the Amyloid Precursor Protein (APP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	H 3
Unit cell lengths	143.990, 143.990, 351.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.000 - 2.700
Rwork	0.215
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mwp 2fjz
RMSD bond length	0.008
RMSD bond angle	1.449
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.066	0.403
Number of reflections	74422
<I/σ(I)>	10.8	2.1
Completeness [%]	99.8
Redundancy	2.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K