3KJ4
Structure of rat Nogo receptor bound to 1D9 antagonist antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-03-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.990 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 188.664, 125.488, 90.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.040 - 3.100 |
| R-factor | 0.25613 |
| Rwork | 0.253 |
| R-free | 0.31775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozn |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.509 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.151 | 0.637 |
| Number of reflections | 37986 | |
| <I/σ(I)> | 9.5 | 1.39 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 14% Peg3350, 0.4M zinc acetate, 0.1M magnesium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
