3K40
Crystal structure of Drosophila 3,4-dihydroxyphenylalanine decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0908 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.809, 108.597, 86.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.880 - 1.750 |
R-factor | 0.19811 |
Rwork | 0.197 |
R-free | 0.22600 |
Structure solution method | MOLREP |
Starting model (for MR) | 1js3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.423 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.060 | 0.560 |
Number of reflections | 100700 | |
Redundancy | 15.1 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15% PEG 4000, 20% glycerol, 200 mM CaCl2, and 100 mM Cacodylic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |