3IN5
Structure of human DNA polymerase kappa inserting dATP opposite an 8-oxoG DNA lesion
Replaces: 3HEDExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.846, 154.480, 217.293 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.120 - 3.200 |
| R-factor | 0.22696 |
| Rwork | 0.225 |
| R-free | 0.27403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.283 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 34388 | |
| <I/σ(I)> | 26.8 | 7.4 |
| Completeness [%] | 100.0 | 99.87 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 14% PEG 5000 monomethyl ether, 0.2M potassium acetate, 0.1M sodium chloride, 0.1M sodium cacodylate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
