3IK9
Human GST A1-1-GIMF with GSDHN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.889, 114.658, 96.239 |
| Unit cell angles | 90.00, 117.76, 90.00 |
Refinement procedure
| Resolution | 39.920 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k3y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.107 | 0.701 |
| Number of reflections | 97225 | |
| <I/σ(I)> | 12.649 | |
| Completeness [%] | 89.6 | 61.6 |
| Redundancy | 4.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 16% PEG mme 5000, 0.1 M HEPES, pH 7.5, and 10% isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
