3II6
Structure of human Xrcc4 in complex with the tandem BRCT domains of DNA LigaseIV.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-28 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.243, 85.982, 111.608 |
| Unit cell angles | 67.34, 82.86, 74.52 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.430 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.044 | 0.370 |
| Number of reflections | 96105 | |
| <I/σ(I)> | 12 | 2.7 |
| Completeness [%] | 97.5 | 96 |
| Redundancy | 2.79 | 2.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100mM Sodium/Potassium phosphate, 15% PEG 8000 MME, 200mM Sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
