3II6
Structure of human Xrcc4 in complex with the tandem BRCT domains of DNA LigaseIV.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-28 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 |
Unit cell lengths | 67.243, 85.982, 111.608 |
Unit cell angles | 67.34, 82.86, 74.52 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.28000 |
Structure solution method | SAD |
Data reduction software | CrystalClear |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.430 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.044 | 0.370 |
Number of reflections | 96105 | |
<I/σ(I)> | 12 | 2.7 |
Completeness [%] | 97.5 | 96 |
Redundancy | 2.79 | 2.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100mM Sodium/Potassium phosphate, 15% PEG 8000 MME, 200mM Sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |