3IGQ
Crystal structure of the extracellular domain of a bacterial pentameric ligand-gated ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.450, 130.240, 113.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.20902 |
| Rwork | 0.207 |
| R-free | 0.25515 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.831 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.057 | 0.424 |
| Number of reflections | 105975 | |
| <I/σ(I)> | 11.23 | 1.96 |
| Completeness [%] | 98.4 | 97.91 |
| Redundancy | 2.03 | 2.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
