3HVO
Structure of the genotype 2B HCV polymerase bound to a NNI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.293, 64.648, 135.714 |
| Unit cell angles | 90.00, 89.73, 90.00 |
Refinement procedure
| Resolution | 19.840 - 2.000 |
| R-factor | 0.18353 |
| Rwork | 0.182 |
| R-free | 0.21823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gsz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.208 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 88994 | |
| <I/σ(I)> | 10.5 | 2.2 |
| Completeness [%] | 98.0 | 93.7 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 300 | 10% PEG4000, 0.1M sodium citrate, 0.2M sodium chloride, 5mM DTT, Protein concentration 6.5mg/ml, pH6.1, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
