3HPG
Visna virus integrase (residues 1-219) in complex with LEDGF IBD: examples of open integrase dimer-dimer interfaces
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9696 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.104, 148.904, 91.076 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 38.672 - 3.280 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2B4J and 1K6Y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.261 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.460 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.110 | 0.665 |
| Number of reflections | 34296 | 5069 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.6 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 291 | 25-30% Jeffamine M600, 100mM Bis-Tris propane-HCl, pH6.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
