3HO5
Crystal structure of Hedgehog-interacting protein (HHIP) and Sonic hedgehog (SHH) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97839 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.618, 101.618, 302.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.010 |
| R-factor | 0.23381 |
| Rwork | 0.231 |
| R-free | 0.28738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1VHH 3ho4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 37106 | |
| <I/σ(I)> | 24.2 | 4.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.7 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1 M bis-Tris propane pH 7.0 and 2.2-2.5 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
