3HME
Crystal structure of human bromodomain containing 9 isoform 1 (BRD9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.264, 124.586, 30.082 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.620 - 2.230 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.459 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.2) |
| Phasing software | PHASER (2.1.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.620 | 2.350 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.083 | 0.598 |
| Number of reflections | 13436 | |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 97.3 | 81.8 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25.5% PEG 3350, 0.17M (NH4)2SO4, 15% glyc, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
