3HEI
Ligand Recognition by A-Class Eph Receptors: Crystal Structures of the EphA2 Ligand-Binding Domain and the EphA2/ephrin-A1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.483, 102.108, 135.255 |
| Unit cell angles | 84.25, 79.77, 73.94 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.1749 |
| Rwork | 0.172 |
| R-free | 0.23299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.049 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.069 | 0.045 | 0.273 |
| Number of reflections | 192014 | ||
| <I/σ(I)> | 18.214 | ||
| Completeness [%] | 96.1 | 99.2 | 91.2 |
| Redundancy | 2.3 | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 8,000, 100 mM Tris, pH 8.5, vapor diffusion, hanging drop, temperature 293K |
