3HAX
Crystal structure of a substrate-bound Gar1-minus H/ACA RNP from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Collection date | 2008-05-29 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 187.809, 63.696, 83.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.110 |
R-factor | 0.20389 |
Rwork | 0.203 |
R-free | 0.22034 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hvy |
RMSD bond length | 0.006 |
RMSD bond angle | 0.959 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.160 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.114 | 0.515 |
Number of reflections | 57734 | |
<I/σ(I)> | 13.7 | 2.7 |
Completeness [%] | 99.6 | 99 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M ammonium acetate, 0.15M magnesium acetate, 4% PEG 8000, 50mM cacodylate sodium, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |