3H4D
Ternary complex of human DNA polymerase iota with template U/T and incoming dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-08-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.92017 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 97.915, 97.915, 203.392 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.680 - 2.200 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2alz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 30091 | |
<I/σ(I)> | 31.4 | 5.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.3 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10-15% PEG 5K MME, 0.2-0.4 M Ammonium sulfate, 0.1 M MES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |