3H32
Crystal structure of D-dimer from human fibrin complexed with Gly-His-Arg-Pro-Tyr-amide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.720, 97.320, 132.490 |
| Unit cell angles | 90.00, 122.78, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.2631 |
| Rwork | 0.263 |
| R-free | 0.31990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fze |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.679 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.600 |
| Number of reflections | 31584 |
| Completeness [%] | 95.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Equal volumes of (a) 5 mg/mL D-dimer, 0.5 mM GHRPYam, 0.3 mM GPRPam, 0.05 M Tris-HCl pH 8.0 and (b) 1% PEG 3350, 1 mM Iodoacetamide, 1 mM CaCl2, 2mM Sodium azide, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
