3GMH
Crystal Structure of the Mad2 Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 124.829, 104.918, 157.746 |
| Unit cell angles | 90.00, 97.57, 90.00 |
Refinement procedure
| Resolution | 49.735 - 3.950 |
| R-factor | 0.2199 |
| Rwork | 0.218 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v64 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.090 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.165 | 0.780 |
| Number of reflections | 35718 | |
| <I/σ(I)> | 11.6 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.6M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
