3GEQ
Structural basis for the chemical rescue of Src kinase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.649, 63.462, 83.860 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.226 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2src |
| RMSD bond length | 0.074 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 31912 | |
| Completeness [%] | 98.7 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 50 mM Ammoniumacetate, 100 mM Bis-Tris pH 5.5, 1 mM DTT, 50 mM imidazole, 0.5 mM PP2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
