3GDH

Methyltransferase domain of human Trimethylguanosine Synthase 1 (TGS1) bound to m7GTP and adenosyl-homocysteine (active form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	H 3
Unit cell lengths	156.248, 156.248, 100.309
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.182
Rwork	0.180
R-free	0.21300
RMSD bond length	0.005
RMSD bond angle	0.914
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.054	0.027	0.359
Number of reflections	61489
<I/σ(I)>	16.464
Completeness [%]	99.8	98.7	100
Redundancy	2.4	2.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	PEG 8000, pH 6, vapor diffusion, temperature 298K