3G8Q
A cytidine deaminase edits C-to-U in transfer RNAs in archaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-25 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.465, 77.060, 109.082 |
| Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.20496 |
| Rwork | 0.202 |
| R-free | 0.26482 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 55130 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | 0.1 M Na cacodylate, 32-40% PEG 400, 0.1 M Ca acetate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
