3G7V
Islet Amyloid Polypeptide (IAPP or Amylin) fused to Maltose Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.025, 96.407, 103.996 |
| Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
| Resolution | 73.920 - 1.860 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mpd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.313 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.054 | 0.035 | 0.460 |
| Number of reflections | 169517 | ||
| <I/σ(I)> | 27.432 | ||
| Completeness [%] | 98.4 | 99.6 | 96.9 |
| Redundancy | 5.5 | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.0 M Ammonium Sulfate, 0.1M Sodium Acetate pH 4.6, vapor diffusion, hanging drop, temperature 298K |
