3G33
Crystal structure of CDK4/cyclin D3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-17 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97650 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 141.297, 141.297, 143.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.190 - 3.000 |
| R-factor | 0.28304 |
| Rwork | 0.281 |
| R-free | 0.31442 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.535 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.830 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.507 |
| Number of reflections | 29147 | |
| <I/σ(I)> | 13.8 | 1.3 |
| Completeness [%] | 98.4 | 94.9 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | 0.1 M Hepes, 15% PEG 3,350, 10% tacsimate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
