3FUB
Crystal structure of GDNF-GFRalpha1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 45.200, 84.100, 179.200 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.22834 |
| Rwork | 0.226 |
| R-free | 0.28021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 30497 | |
| <I/σ(I)> | 2.95 | |
| Completeness [%] | 97.0 | 91.3 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 15% PEG4000, 0.15 M (NH4)2SO4, 0.1 M MES buffer pH 6, Vapor diffusion, temperature 293K |
