3FRT
The structure of human CHMP3 (residues 8 - 222).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97607 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.472, 131.514, 48.508 |
| Unit cell angles | 90.00, 108.06, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.000 |
| R-factor | 0.388 |
| Rwork | 0.383 |
| R-free | 0.43600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gd5 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.160 | 0.439 |
| Number of reflections | 2950 | |
| <I/σ(I)> | 9.33 | 2.92 |
| Completeness [%] | 78.7 | 78.7 |
| Redundancy | 4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 286 | 10-22% PEG 6000 0.1 M Hepes, pH 7.0-8.0, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
