3FNV
Crystal Structure of Miner1: The Redox-active 2Fe-2S Protein Causative in Wolfram Syndrome 2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.7418, 1.3624, 1.7372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.904, 48.579, 74.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.060 - 2.100 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21600 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.859 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.143 | 1.790 |
| Number of reflections | 14086 | 1285 |
| <I/σ(I)> | 16.17 | 1.9 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 25.9 | 17.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 300 | 100 mM Tris-HCl pH 8.0, 100 mM NaCl, 15% PEG 3000, VAPOR DIFFUSION |
