3FMZ
Crystal Structure of Retinol-Binding Protein 4 (RBP4) in complex with non-retinoid ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.802, 84.802, 119.557 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.28600 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.521 | 119.560 | 3.060 |
| High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
| Rmerge | 0.125 | 0.043 | 0.402 |
| Total number of observations | 1312 | 6012 | |
| Number of reflections | 10890 | ||
| <I/σ(I)> | 5.773 | 13.4 | 1.9 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 3.7 | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 0.4M Mg formate, 0.1M GndHCl, 0.1M Bis-Tris 6.5, vapor diffusion, temperature 289K |
