3FK7
Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9082 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.220, 59.380, 63.780 |
| Unit cell angles | 90.00, 97.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.060 |
| R-factor | 0.208 |
| Rwork | 0.203 |
| R-free | 0.26100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3fk6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.220 | 2.190 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.060 | 0.398 |
| Number of reflections | 29597 | |
| Completeness [%] | 98.6 | 91.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 1M dipotassium hydrogen phosphate, 200mM sodium chloride, 50mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
