3FK7

Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tetracycline repressor protein class B from transposon Tn10, Tetracycline repressor protein class D	polymer	208	23448.8	2	UniProt (P04483) UniProt (P0ACT4) Pfam (PF00440) Pfam (PF02909)	Escherichia coli
2	C, E (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, F (A, B)	(4aS,12aS)-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4a,5,12,12a-hexahydrotetracene-2-carboxamide	non-polymer		383.4	2	Chemie (4DM)
4	G, H (A, B)	water	water		18.0	225	Chemie (HOH)

Sequence modifications

A, B: 1 - 50 (UniProt: P04483)
A, B: 51 - 208 (UniProt: P0ACT4)

PDB	External Database	Details
Lys 64	His 64	engineered mutation
Leu 135	Ser 135	engineered mutation
Ile 138	Ser 138	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46897.6
Non-Polymers*	Number of molecules	4
	Total formula weight	815.3
All*	Total formula weight	47712.9

*Water molecules are not included.