3FEA
Crystal Structure of HdmX bound to the p53-peptidomimetic Ac-Phe-Met-Aib-Pmp-6-Cl-Trp-Glu-Ac3c-Leu-NH2 at 1.33A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98371 |
Spacegroup name | P 41 |
Unit cell lengths | 42.249, 42.249, 70.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.330 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fe7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.584 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.5.0039) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.380 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.062 | 0.266 |
Number of reflections | 27490 | |
<I/σ(I)> | 39.165 | |
Completeness [%] | 97.1 | 74.2 |
Redundancy | 8.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 3.1M AmSo4, 1% MPD, 0.1M MES, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |