3FCS
Structure of complete ectodomain of integrin aIIBb3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 41 |
| Unit cell lengths | 81.300, 81.300, 654.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 2.550 |
| R-factor | 0.23332 |
| Rwork | 0.233 |
| R-free | 0.26802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Combination of PDB ENTRY 1JV2 and 1TXV |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.741 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.071 | 0.566 |
| Number of reflections | 135066 | |
| <I/σ(I)> | 12.17 | 2.13 |
| Completeness [%] | 98.6 | 93.9 |
| Redundancy | 4.55 | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 10% PEG 3350, 50 mM magnesium acetate, and 0.1 M imidazole, pH 7.0, VAPOR DIFFUSION, temperature 298.0K |
