3F8J
Mouse UHRF1 SRA domain bound with hemi-methylated CpG, crystal structure in space group C222(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.670, 91.590, 118.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.910 - 1.990 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zo0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.910 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.102 | 0.568 |
| Number of reflections | 23488 | |
| <I/σ(I)> | 9 | 3.6 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 9.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 20% PEG 3350, 0.4 M NaCl, pH 7.0, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 2 | PEG 3350 | ||
| 4 | 1 | 2 | NaCl |
