3F1N
Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with internally bound ethylene glycol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-13 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.321, 82.908, 41.400 |
| Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
| Resolution | 30.972 - 1.479 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b02 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 1.490 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.042 | 0.045 |
| Number of reflections | 39281 | |
| <I/σ(I)> | 32.6 | 1.9 |
| Completeness [%] | 99.1 | 91.3 |
| Redundancy | 4.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion combined with microseeding | 6.5 | 293 | 30% PEG 3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT, pH 6.5, vapor diffusion combined with microseeding, temperature 293K |
