3EZQ
Crystal Structure of the Fas/FADD Death Domain Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Collection date | 2007-08-30 |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 126.218, 126.218, 299.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.730 |
| R-factor | 0.2361 |
| Rwork | 0.234 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.279 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.801 |
| High resolution limit [Å] | 2.730 | 2.731 |
| Rmerge | 0.076 | 0.435 |
| Number of reflections | 66820 | |
| <I/σ(I)> | 9.3 | 1.7 |
| Completeness [%] | 98.7 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 291 | 0.95M citric acid, 1.9M ammonium sulfate, pH4, EVAPORATION, temperature 291K |
