3EQ1
The Crystal Structure of Human Porphobilinogen Deaminase at 2.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.083, 104.435, 109.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.901 - 2.800 |
| R-factor | 0.2615 |
| Rwork | 0.259 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pda |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.658 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.103 | 0.657 |
| Number of reflections | 25917 | |
| <I/σ(I)> | 11.9 | 2.9 |
| Completeness [%] | 95.6 | 96.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.6M ammonium sulphate, 1.2M lithium sulphate, 5% (v/v) ethylene glycol, 50mM sodium citrate, 50mM DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
