3EMW
Crystal Structure of human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2) in complex with a 20-residue VASA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.975653 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.470, 61.963, 118.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.830 - 1.800 |
| R-factor | 0.17995 |
| Rwork | 0.178 |
| R-free | 0.21593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.618 |
| Number of reflections | 24366 | |
| <I/σ(I)> | 15 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.178M Na/KPO4, 17.8% PEG3350, 8.88% EtGly, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
